Application
Western blot analysis of INPP5D (arrow) using rabbit polyclonal INPP5D Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the INPP5D gene (Lane 2)| Product Name | INPP5D antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to INPP5D (Internal)This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. Overall, the protein functions as a negative regulator of myeliod cell proliferation and survival. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 776~805 amino acids from the Center region of human INPP5D. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| ELISA: Use at 1:1,000: |
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| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | hp51CN SH2 domaincontaining inositol5'phosphatase 1 SH2 domain-containin SHIP SIP145 SH2 domaincontainin Phosphatidylinositol-3 4 5-trisphosphate 5-phosphatase 1 Phosphatidylinositol3 4 5trisphosphate5phosphatase1 SH2 domaincontaining inositol phos |
|---|---|
| Target | INPP5D |
| Molecular Weight(MW) | 133.292 kDa (note) |
| Cellular Localization | Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Partly translocated via its SH2 domain which mediates interaction with tyrosine phosphorylated receptors suc |
Application
Western blot analysis of INPP5D (arrow) using rabbit polyclonal INPP5D Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the INPP5D gene (Lane 2)| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
