Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Hela cells using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody and the same antibody preincubated with blocking peptide.Application
Immunofluorescence staining of methanol-fixed Hela cells using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody .| Product Name | PTEN (phospho Ser380/Thr382/Thr383) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PTEN (Phospho Ser380/Thr382/Thr383)Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 380/382/383 (R-Y-S(p)-D-T(p)-T(p)-D-S) derived from Human PTEN. |
| Modification | p-Ser380/Thr382/Thr383 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 10q23del BZS DEC GLM2 MGC11227 MHAM MMAC1 PTEN1 TEP1 PTEN mutated in multiple advanced cancers 1 phosphatase and tensin-like protein phosphatidylinositol-3 4 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN MMAC1 pho |
|---|---|
| Molecular Weight(MW) | 54 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Hela cells using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody and the same antibody preincubated with blocking peptide.
Application
Immunofluorescence staining of methanol-fixed Hela cells using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
