Application
Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 μg/mL.Application
Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 μg/ml.Application
Western blot analysis of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 μg/mL.| Product Name | DOK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DOK1 Antibody: TP1, TLP1, p240, TROVE1, VAULT2, Docking protein 1, Downstream of tyrosine kinase 1 |
| Product description | DOK1 Antibody: Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok.1) Carpino N, Wisniewski D, Strife A, Marshak D, Kobayashi R, Stillman B, Clarkson B p62(dok): a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells. Cell 1997;88:197-204. |
| Immunogen | DOK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DOK1. The immunogen is located within amino acids 410 - 460 of DOK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| DOK1 antibody can be used for detection of DOK1 expression by Western blot at 1 μg/mL. A 62 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TEP1 |
|---|---|
| Alternative Names | DOK1 Antibody: TP1 TLP1 p240 TROVE1 VAULT2 Docking protein 1 Downstream of tyrosine kinase 1 |
| Molecular Weight(MW) | 62 kDa |
| Sequence Similarities | 4) Cat. No. 17-004 - K-562 Cell Slide |
Application
Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 μg/mL.
Application
Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 μg/ml.
Application
Western blot analysis of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | DOK1 antibody can be used for detection of DOK1 expression by Western blot at 1 μg/mL. A 62 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DOK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DOK1 Antibody is supplied in PBS containing 0.02% sodium azide. |
