Application
Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using anti-PTEN mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Application
Flow cytometric analysis of Hela cells using anti-PTEN mAb (right) and negative control (left).Application
Western blot analysis using anti-PTEN monoclonal antibody against Hela (1) and NIH/3T3 (2) cell lysate.| Product Name | PTEN antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1B8] to PTENPTEN (phosphatase and tensin homolog) was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. This protein is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. |
| Immunogen | Purified recombinant fragment of PTEN expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1B8 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| Western Bloting: 1/500 - 1/2000. : |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Synonyms | TEP1 PTEN1 MHAM BZS |
|---|---|
| Target | PTEN |
| Molecular Weight(MW) | 54kDa (note) |
Application
Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using anti-PTEN mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Application
Flow cytometric analysis of Hela cells using anti-PTEN mAb (right) and negative control (left).
Application
Western blot analysis using anti-PTEN monoclonal antibody against Hela (1) and NIH/3T3 (2) cell lysate.| Application Notes | Western Bloting: 1/500 - 1/2000. : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide |
