Application
Western blot analysis of TP1 in human kidney tissue lysate with TP1 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of TP-1 in Human Lung cells with TP-1 antibody at 20 μg/mL.Application
Immunohistochemical staining of human lung tissue using TP1 antibody at 2.5 μg/mL.| Product Name | TP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TP1 Antibody: TP1, TLP1, p240, TROVE1, VAULT2, TP1, Telomerase protein component 1, Telomerase-associated protein 1, Telomerase protein 1 |
| Product description | TP1 Antibody: Telomerase is an RNA-dependent DNA polymerase that uses an RNA component to add telomeric repeat sequences at the ends of chromosomes. Besides the RNA component which serves as the template that specifies the telomeric repeat, the telomerase complex contains a reverse transcriptase protein (TRT) and various accessory proteins including the telomerase-associated protein 1 (TP1). Telomerase activity is low in most somatic cells, causing the gradual shortening of telomeres which can ultimately lead to telomere fusion and cell death. High levels of telomerase activity are widely seen in cancerous cells and while recent experiments have suggested that telomerase may be a viable target in cancer therapy, expression levels of TP1 do not correlate with malignancy. At least two isoforms of TP1 are known to exist.1) Greider CW. Telomere length regulation. Annu. Rev. Biochem.1996; 274:92-7. |
| Immunogen | TP1 antibody was raised against a 20 amino acid synthetic peptide from near the amino terminus of human TP1. The immunogen is located within the first 50 amino acids of TP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| TP1 antibody can be used for detection of TP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TEP1 |
|---|---|
| Alternative Names | TP1 Antibody: TP1 TLP1 p240 TROVE1 VAULT2 TP1 Telomerase protein component 1 Telomerase-associated protein 1 Telomerase protein 1 |
| Molecular Weight(MW) | Predicted: 290 kDa Observed: 290 kDa |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
Application
Western blot analysis of TP1 in human kidney tissue lysate with TP1 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of TP-1 in Human Lung cells with TP-1 antibody at 20 μg/mL.
Application
Immunohistochemical staining of human lung tissue using TP1 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | TP1 antibody can be used for detection of TP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
