Application
Staining of formalin-fixed, paraffin-embedded human pancreas tissue sections with 1:1,000 Monoclonal Anti-Insulin Clone: K36AC10 (Cat. No. ) using biotin/ExtrAvidin-Peroxidase.| Product Name | Insulin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal to InsulinMonoclonal Anti-Insulin may be used for the localization of insulin using RIA, dot blot and immunocytochemistry. Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B or b-cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma. Insulin-specific antibodies prove useful as b-cell and tumor markers using immunohistochemical techniques, and as analytical tools in quantification of the hormone. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | K36AC10 |
| Host Species | Mouse |
| Tested Applications | DotIHC-PRIA |
| Dot: Use at an assay dependant dilution. IHC-P: Use at a dilution of 1/1000. RIA: Use at an assay dependant dilution. Binds to insulin with an affinity constant of 8.8 ? 10^9 M^-1 Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanDogPigRatCatGuinea PigHorseRabbitSheepBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IDDM2 ILPR IRDN MODY10 |
|---|---|
| Target | Insulin |
| Tissue Specificity | The antibody exhibits cross-reactivity with human proinsulin (ref 3). |
| Cellular Localization | Secreted |
Application
Staining of formalin-fixed, paraffin-embedded human pancreas tissue sections with 1:1,000 Monoclonal Anti-Insulin Clone: K36AC10 (Cat. No. ) using biotin/ExtrAvidin-Peroxidase.| Application Notes | Dot: Use at an assay dependant dilution. IHC-P: Use at a dilution of 1/1000. RIA: Use at an assay dependant dilution. Binds to insulin with an affinity constant of 8.8 ? 10^9 M^-1 Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 15 mM Na azide |
