| Product Name | Insulin Receptor (phospho Tyr972) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Insulin Receptor ( phospho Y972 )Biological actions of insulin are mediated by the Insulin Receptor (IR), a receptor tyrosine kinase that regulates multiple signaling pathways through activation of a series of phosphorylation cascades. The IR is a heterotetrameric protein consisting of two ligand-binding alpha subunits and two beta subunits that each contain a tyrosine kinase domain. Insulin binding to the extracellular domain leads to autophosphorylation of the receptor and activation of the intrinsic tyrosine kinase activity, which allows appropriate substrates to be phosphorylated. Tyrosine 972 is in the juxtamembrane Asn-Pro- Glu-Tyr (NPEY) motif. Phosphorylation of IR tyrosine 972 is required for the binding and/or phosphorylation of the adapter protein Shc, the PTB domain, IRS-1, PI3 kinase, and the Suppressor of Cytokine Signaling (SOCS). |
| Immunogen | Synthetic phosphopeptide derived from the region of the human Insulin Receptor that contains tyrosine 972. |
| Modification | p-Tyr972 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1/1000. Detects a band of approximately 110 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Insulin receptor (phospho Y972) Insulin receptor (phospho Tyr972) Insulin receptor (pY972) Insulin receptor (pTyr972) Insulin receptor phospho Y972 Insulin receptor phospho Tyr972 Phospho Insulin receptor (Y972) Phospho Insulin receptor (Tyr972) Ph |
|---|---|
| Target | Insulin receptor |
| Cellular Localization | Plasma membrane |
| Positive Control | CHO-T cells transfected with a vector encoding the human insulin receptor and stimulated with insulin, and 3T3-L1 adipocytes +/- insulin stimulation |
|---|---|
| Application Notes | WB: 1/1000. Detects a band of approximately 110 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA |
