Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor antibody diluted at 1:1000.Application
Insulin Receptor antibody detects Insulin Receptor protein at membrane on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Insulin Receptor antibody dilution: 1:500.Application
Insulin Receptor antibody detects INSR protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. Insulin Receptor antibody dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Insulin Receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Insulin Receptor (insulin receptor)After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Insulin Receptor. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | RatRat (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD220 antibody HHF5 antibody INSR antibody IR antibody insulin receptor antibody |
|---|---|
| Molecular Weight(MW) | 156 kDa (note) (2) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Insulin Receptor antibody diluted at 1:1000.
Application
Insulin Receptor antibody detects Insulin Receptor protein at membrane on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Insulin Receptor antibody dilution: 1:500.
Application
Insulin Receptor antibody detects INSR protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. Insulin Receptor antibody dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
