Application
Western blot analysis of extract from JK and Raji cells using LPAM-1(Integrin a4, CD49d) Antibody| Product Name | Integrin alpha 4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Integrin alpha 4Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. |
| Immunogen | Peptide sequence around aa.1024~1028(Y-I-N-S-K) derived from Human LPAM-1(Integrin alpha 4, CD49d). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD49D IA4 MGC90518 ITGA4 VLA-4 subunit alpha very late activation protein 4 receptor alpha 4 subunit antigen CD49D alpha-4 subunit of VLA-4 receptor integrin alpha-4 integrin alpha-4 subunit CD49 antigen-like family member D integrin alpha 4 2 |
|---|---|
| Target | Integrin alpha 4 |
| Molecular Weight(MW) | 70, 140, 180 (note) |
Application
Western blot analysis of extract from JK and Raji cells using LPAM-1(Integrin a4, CD49d) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
