| Product Name | Integrin alpha 6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MP 4F10] to Integrin alpha 6Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around twentytwo different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell-surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N-terminal extracellular domain that binds the ECM ligand and a small C-terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. Integrin alpha 6 complexes are receptors for laminins, which are componants of basement membranes. Integrin alpha 6 complexes may play an important role in embryogenesis. |
| Immunogen | Cell preparation: SW1222 human colorectal cell line |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | MP 4F10 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FIP |
| FACS: Use at an assay dependent dilution. IHC-F: Use at an assay dependent dilution. Note: No reaction on fixed formalin tissue. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | integrin alpha6 VLA6 alpha subunit ITGA6 alpha 6 CD49f alpha6 VLA 6 alpha subunit Integrin alpha 6 integrin alpha chain DKFZp686J01244 FLJ18737 ITGA6B VLA-6 VLA 6 VLA6 |
|---|---|
| Target | Integrin alpha 6 |
| Tissue Specificity | Reacts with alpha 6. MP 4F10 can be used in clinical studies for tumour grading - stains moderately and well-differentiated colorectal adenocarcinoma. |
| Cellular Localization | Cell Membrane |
| Application Notes | FACS: Use at an assay dependent dilution. IHC-F: Use at an assay dependent dilution. Note: No reaction on fixed formalin tissue. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.1% sodium azide |
