Application
Flow cytometry analysis of CD29 in peripheral blood granulocytes compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and CD29 antibody [TS2/16] at a dilution of 0.5 ug/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a proper secondary. FACS analysis was performed using 400 ul of cell buffer.| Product Name | Integrin beta 1 / CD29 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [TS2/16] to Integrin beta 1 / CD29Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Human Beta1 Integrin, the Beta1 chain of the Beta1 integrin heterodimer (CD29) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | TS2/16 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIPWBFunctional Assay |
| FACS: 0.5 ug/test Optimal dilution / concentration should be determined by the end user.: |
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| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | GPIIA VLA-BETA ITGB1 VLAB integrin beta 1 (fibronectin receptor beta polypeptide antigen CD29 includes MDF2 MSK12) MDF2 FNRB CD29 MSK12 |
|---|---|
| Target | Integrin beta 1 / CD29 |
Application
Flow cytometry analysis of CD29 in peripheral blood granulocytes compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and CD29 antibody [TS2/16] at a dilution of 0.5 ug/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a proper secondary. FACS analysis was performed using 400 ul of cell buffer.| Application Notes | FACS: 0.5 ug/test Optimal dilution / concentration should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | -20°C |
| Storage Buffer | PBS |
