Application
IHC image using - Detection of Integrin beta 3 by IHC in human bone marrow| Product Name | Integrin beta 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [Y2/51] to Integrin beta 3This antibody recognizes a glycoprotein of 110 kD molecular weight present on human platelets and also reacts with megakaryocytes and megakaryoblasts. The antigen is also present on endothelium and osteoclasts. The glycoprotein IIIa molecule produced by endothelium cells is identical in sequence to platelet glycoprotein IIIa. |
| Immunogen | PHA-stimulated human mononuclear cells |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | Y2/51 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-P |
| For IHC-P: Use at a dilution of 1:25 - 1:50 in an ABC method. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. We suggest an incubation period of 30 minutes at room temperature. This antibody is not compatible with Zenker and B5 fixed tissues. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD61 GP3A Integrin beta 3 Integrin beta3 GPIIIa 3690 ITGB3 P05106 173470 |
|---|---|
| Target | Integrin beta 3 |
| Cellular Localization | Cell membrane |
| Light Chain Type | Kappa |
Application
IHC image using - Detection of Integrin beta 3 by IHC in human bone marrow| Positive Control | Tonsil |
|---|---|
| Application Notes | For IHC-P: Use at a dilution of 1:25 - 1:50 in an ABC method. Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining. We suggest an incubation period of 30 minutes at room temperature. This antibody is not compatible with Zenker and B5 fixed tissues. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2 containing 0.05% sodium azide |
