Application
Human Small Intestine stained with anti-Integrin beta 5 antibody.Application
Human Small Intestine stained with anti-Integrin beta 5 antibody| Product Name | Integrin beta 5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Integrin beta 5Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around 22 different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell-surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in-groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N-terminal extracellular domain that binds the ECM ligand and a small C-terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. |
| Immunogen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) C terminal (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| IF: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/50. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate, pH 6.0 for 10 min followed by cooling at RT for 20 min. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 95.9 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatHamster |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Integrin beta5 ITGB5 Integrin beta 5 FLJ26658 |
|---|---|
| Target | Integrin beta 5 |
| Cellular Localization | Cell Membrane and Cytoplasmic |
Application
Human Small Intestine stained with anti-Integrin beta 5 antibody.
Application
Human Small Intestine stained with anti-Integrin beta 5 antibody| Positive Control | Small intestine, bone marrow and colon carcinoma |
|---|---|
| Application Notes | IF: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/50. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate, pH 6.0 for 10 min followed by cooling at RT for 20 min. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 95.9 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide |
