Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using IP6K1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded Ca922 Xenograft, using IP6K1 antibody at 1:500 dilution.Application
IP6K1 antibody detects IP6K1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human IHPK1-transfected 293T cells 10% SDS-PAGE IP6K1 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
IP6K1 antibody detects IP6K1 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg THP-1 whole cell lysate/extract D. 30 µg NCI-H929 whole cell lysate/extract 10% SDS-PAGE IP6K1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | IP6K1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IP6K1 (inositol hexakisphosphate kinase 1)This gene encodes a protein that belongs to the inositol phosphokinase (IPK) family. This protein is likely responsible for the conversion of inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). It may also convert 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4. Alternative splicing occurs for this gene; however, the full-length nature of all transcript variants has not yet been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IP6K1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (96%), Rhesus Monkey (99%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IHPK1 antibody MGC9925 antibody PiUS antibody IP6K1 antibody insP6 kinase 1 antibody inositol hexakisphosphate kinase 1 antibody ATP:1D-myo-inositol-hexakisphosphate phosphotransferase antibody Pi uptake stimulator antibody inositol hexaphosphate k |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (2) |
| Tissue Specificity | Knockdown/Knockout validation was supported by references (PMID:27701146) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using IP6K1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded Ca922 Xenograft, using IP6K1 antibody at 1:500 dilution.
Application
IP6K1 antibody detects IP6K1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human IHPK1-transfected 293T cells 10% SDS-PAGE IP6K1 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
IP6K1 antibody detects IP6K1 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg THP-1 whole cell lysate/extract D. 30 µg NCI-H929 whole cell lysate/extract 10% SDS-PAGE IP6K1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Jurkat , Raji , THP-1, NCI-H929 , human IHPK1-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
