Application
Western blot analysis of extracts of various cell lines, using IPO5 antibody.Application
Immunohistochemistry of paraffin-embedded mouse lung using IPO5 antibody at dilution of 1:100 (x400 lens).Application
Immunofluorescence analysis of U2OS cell using IPO5 antibody. Blue: DAPI for nuclear staining.Application
Immunofluorescence analysis of A549 cell using IPO5 antibody. Blue: DAPI for nuclear staining.| Product Name | IPO5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IPO5Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human IPO5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IMB3 importin 5 RANBP5 Pse1 KPNB3 imp5 |
|---|---|
| Target | IPO5 |
| Molecular Weight(MW) | 124 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using IPO5 antibody.
Application
Immunohistochemistry of paraffin-embedded mouse lung using IPO5 antibody at dilution of 1:100 (x400 lens).
Application
Immunofluorescence analysis of U2OS cell using IPO5 antibody. Blue: DAPI for nuclear staining.
Application
Immunofluorescence analysis of A549 cell using IPO5 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
