Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IPO5 antibody at 1:500 dilution.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: Hela 5% SDS PAGE diluted at 1:2000| Product Name | IPO5 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IPO5 (importin 5)Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human IPO5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Xenopus laevis, Pig, Chimpanzee, BovineXenopus laevis (81%), Pig (97%), Chimpanzee (100%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686O1576 antibody FLJ43041 antibody IMB3 antibody KPNB3 antibody MGC2068 antibody RANBP5 antibody IPO5 antibody importin beta-3 subunit antibody karyopherin (importin) beta 3 antibody Ran_GTP binding protein 5 antibody RAN binding protein 5 |
|---|---|
| Molecular Weight(MW) | 124 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus , nucleolus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IPO5 antibody at 1:500 dilution.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: Hela 5% SDS PAGE diluted at 1:2000| Positive Control | A549 , NT2D1 , HeLa , Jurkat , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
