Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using IRAK4 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:1000Application
IRAK4 antibody detects IRAK4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: IRAK4 protein stained by IRAK4 antibody diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | IRAK4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IRAK4 (interleukin-1 receptor-associated kinase 4)This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [supplied by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IRAK4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Pig, Sheep, Rhesus Monkey, BovinePig (82%), Sheep (85%), Rhesus Monkey (97%), Bovine (84%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IPD1 antibody NY-REN-64 antibody REN64 antibody IRAK4 antibody interleukin-1 receptor-associated kinase 4 antibody renal carcinoma antigen NY-REN-64 antibody |
|---|---|
| Molecular Weight(MW) | 52 kDa (note) (3) |
Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using IRAK4 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:1000
Application
IRAK4 antibody detects IRAK4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: IRAK4 protein stained by IRAK4 antibody diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
