Application
Untreated (–) and treated (+) HepG2 whole cell extracts (20 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 (phospho Ser724) antibody diluted at 1:1000.| Product Name | IRE1 alpha (phospho Ser724) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IRE1 alpha (phospho Ser724) (endoplasmic reticulum to nucleus signaling 1)The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human IRE1 (phospho Ser724). The exact sequence is proprietary. |
| Modification | p-Ser724 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIHCWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMousePorcineRat |
| Predicted Reactivity | Mouse, Rat, Japanese medakaMouse (100%), Rat (100%), Japanese medaka (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | IRE1 alpha |
|---|---|
| Molecular Weight(MW) | 110 kDa (note) |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein |
Application
Untreated (–) and treated (+) HepG2 whole cell extracts (20 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 (phospho Ser724) antibody diluted at 1:1000.| Positive Control | HepG2 , HepG2 (3μM Thapsigargin treatment for 12 hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
