Application
Western blot analysis on 293T cell lysates using anti-Phospho-IRE1 (pS724) RabMAb (cat. #). Cells were either (A) untreated (B) treated with Calyculin A.| Product Name | IRE1 alpha (phospho Ser724) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR5253] to IRE1 alpha (phospho Ser724)IRE1 is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. It possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering protein expression as a response to endoplasmic reticulum-based stress signals (1). |
| Immunogen | A phospho specific peptide corresponding to residues surrounding Serine 724 of human IRE1 was used as an immunogen. This antibody only detects IRE1 phosphorylated at Serine 724. |
| Modification | p-Ser724 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR5253 |
| Host Species | Rabbit |
| Tested Applications | WB |
| Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Target | IRE1 alpha (phospho Ser724) |
|---|
Application
Western blot analysis on 293T cell lysates using anti-Phospho-IRE1 (pS724) RabMAb (cat. #). Cells were either (A) untreated (B) treated with Calyculin A.| Application Notes | Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
