Application
IRE1 alpha antibody detects IRE1 alpha protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 alpha antibody diluted by 1:1000.| Product Name | IRE1 alpha antibody , N-term |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to IRE1 alpha (endoplasmic reticulum to nucleus signaling 1)The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human IRE1 alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Japanese medakaMouse (99%), Rat (98%), Zebrafish (83%), Japanese medaka (86%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ30999 antibody IRE1 antibody IRE1P antibody IRE1a antibody MGC163277 antibody MGC163279 antibody hIRE1p antibody ERN1 antibody serine/threonine-protein kinase/endoribonuclease IRE1 antibody inositol-requiring protein 1 antibody ER to nucleus s |
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| Target | IRE1 alpha |
| Molecular Weight(MW) | 110 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein |
Application
IRE1 alpha antibody detects IRE1 alpha protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 alpha antibody diluted by 1:1000.| Positive Control | 293T , A431 , HeLa , HepG2 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
