Application
IRF3 (phospho Ser386) antibody detects IRF3 (phospho Ser386) protein by western blot analysis. Un-treated (-) and treated (+, CIP treatment for 1hr) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IRF3 (phospho Ser386) antibody at a dilution of 1:5000.| Product Name | IRF3 (phospho Ser386) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IRF3 (phospho Ser386) (interferon regulatory factor 3)IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. [provided by RefSeq] |
| Immunogen | carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human IRF3 (phospho Ser386). The exact sequence is proprietary. |
| Modification | p-Ser386 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IRF3 phospho S386 IRF3 (pS386) phospho IRF3 phospho IRF3 (S386) IRF3 phospho Ser386 IRF3 phospho phospho Ser386 IRF3 phospho IRF3 (pS386) IRF3 (phospho Ser386) IRF3 (phospho S386) IRF3 (pSer386) interferon regulatory factor |
|---|---|
| Target | IRF3 (phospho Ser386) |
| Molecular Weight(MW) | 47 kDa (note) (7) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
IRF3 (phospho Ser386) antibody detects IRF3 (phospho Ser386) protein by western blot analysis. Un-treated (-) and treated (+, CIP treatment for 1hr) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IRF3 (phospho Ser386) antibody at a dilution of 1:5000.| Positive Control | HeLa , HeLa treat with CIP |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
