Application
Western blot analysis of IRF4 in Jurkat cell lysate with IRF4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 ug/mL.Application
Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 ug/mL.| Product Name | IRF4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IRF4The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2010] |
| Immunogen | IRF4 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human IRF4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| IF: 20 ug/mL: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | SHEP8 MUM1 NF-EM5 LSIRF interferon regulatory factor 4 |
|---|---|
| Target | IRF4 |
| Tissue Specificity | IRF4 antibody is predicted to not cross-react with other IRF protein family members. IRF4 often migrates at a higher than predicted molecular weight in SDS-PAGE. |
Application
Western blot analysis of IRF4 in Jurkat cell lysate with IRF4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 ug/mL.
Application
Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 ug/mL.| Application Notes | IF: 20 ug/mL: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4ºC, stable for one year. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
