Application
IHC-P analysis of human normal colon and colon adenocarcinoma tissue using IRF5 antibody. Heat mediated antigen retrieval using citrate buffer pH6.0.Application
WB analysis of Ramos, THP-1, A20, and NIH3T3 cell lysate using IRF5 antibody [10T1].| Product Name | IRF5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [10T1] to IRF5This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Mar 2010] |
| Immunogen | Histidine-tagged recombinant peptide corresponding to amino acids 176-240 of human IRF5 purified from E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 10T1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-PIPWB |
| Western Blotting: 1/1000 - 1/2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SLEB10 interferon regulatory factor 5 |
|---|---|
| Target | IRF5 |
| Tissue Specificity | This antibody recognizes human interferon regulatory factor 5 (IRF5). |
Application
IHC-P analysis of human normal colon and colon adenocarcinoma tissue using IRF5 antibody. Heat mediated antigen retrieval using citrate buffer pH6.0.
Application
WB analysis of Ramos, THP-1, A20, and NIH3T3 cell lysate using IRF5 antibody [10T1].| Application Notes | Western Blotting: 1/1000 - 1/2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.1% Sodium Azide (NaN3) |
