Application
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using ISG20 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ISG20 antibody at 1:500 dilution.| Product Name | ISG20 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ISG20 (interferon-stimulated gene 20 kDa protein)Exonuclease with specificity for single-stranded RNA and, to a lesser extent for DNA. Degrades RNA at a rate that is approximately 35-fold higher than its rate for single-stranded DNA. Involved in the antiviral function of IFN against RNA viruses. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ISG20. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rhesus MonkeyMouse (83%), Rat (82%), Pig (95%), Rhesus Monkey (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD25 antibody HEM45 antibody ISG20 antibody estrogen-regulated transcript 45 protein antibody promyelocytic leukemia nuclear body-associated protein ISG20 antibody interferon-stimulated gene 20 kDa protein antibody interferon stimulated exonuclease g |
|---|---|
| Molecular Weight(MW) | 20 kDa (note) (5) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using ISG20 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ISG20 antibody at 1:500 dilution.| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
