Application
Sample(30 μg of whole cell lysate) A:293T B:H1299 7.5% SDS PAGE diluted at 1:1500Application
ITK antibody [C3], C-term detects ITK protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ITK protein stained by ITK antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | ITK antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ITK (IL2-inducible T-cell kinase)This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ITK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EMT antibody LYK antibody MGC126257 antibody MGC126258 antibody PSCTK2 antibody ITK antibody kinase EMT antibody tyrosine-protein kinase LYK antibody T-cell-specific kinase antibody tyrosine-protein kinase ITK/TSK antibody homolog of mouse T-cell |
|---|---|
| Target | ITK |
| Molecular Weight(MW) | 72 kDa (note) |
| Cellular Localization | Cell membrane (By similarity) |
Application
Sample(30 μg of whole cell lysate) A:293T B:H1299 7.5% SDS PAGE diluted at 1:1500
Application
ITK antibody [C3], C-term detects ITK protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ITK protein stained by ITK antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , H1299 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
