Application
Immunofluorescence analysis of methanol-fixed HeLa, using ITM2B antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: Hep G2 B: HCT116 12% SDS PAGE diluted at 1:1000Application
ITM2B antibody detects ITM2B protein by Western blot analysis. A.50 µg mouse brain lysate/extract 12 % SDS-PAGE ITM2B antibody dilution: 1:1000Application
ITM2B antibody detects ITM2B protein at Golgi apparatus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain stem. ITM2B antibody dilution: 1:500.| Product Name | ITM2B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ITM2B (integral membrane protein 2B)Amyloid precursor proteins are processed by beta-secretase and gamma-secretase to produce beta-amyloid peptides which form the characteristic plaques of Alzheimer disease. This gene encodes a transmembrane protein which is processed at the C-terminus by furin or furin-like proteases to produce a small secreted peptide which inhibits the deposition of beta-amyloid. Mutations which result in extension of the C-terminal end of the encoded protein, thereby increasing the size of the secreted peptide, are associated with two neurogenerative diseases, familial British dementia and familial Danish dementia. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ITM2B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Sheep, Rhesus Monkey, Chimpanzee, BovineRat (96%), Pig (95%), Sheep (96%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ABRI antibody BRI antibody BRI2 antibody BRICD2B antibody E25B antibody E3-16 antibody FBD antibody ITM2B antibody BRICHOS domain containing 2B antibody integral membrane protein 2B antibody transmembrane protein BRI antibody ABri/ADan amyloid p |
|---|---|
| Molecular Weight(MW) | 30 kDa (note) (2) |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using ITM2B antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 B: HCT116 12% SDS PAGE diluted at 1:1000
Application
ITM2B antibody detects ITM2B protein by Western blot analysis. A.50 µg mouse brain lysate/extract 12 % SDS-PAGE ITM2B antibody dilution: 1:1000
Application
ITM2B antibody detects ITM2B protein at Golgi apparatus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain stem. ITM2B antibody dilution: 1:500.| Positive Control | HepG2 , HCT116 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
