Application
Immunofluorescence analysis of methanol-fixed HeLa, using IVD antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using IVD antibody at 1:250 dilution.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000Application
IVD antibody detects IVD protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10 % SDS-PAGE IVD antibody dilution: 1:1000Application
Sample (30 ug of whole cell lysate) A: Hela B: JurKat 10% SDS PAGE diluted at 1:1000| Product Name | IVD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IVD (isovaleryl-CoA dehydrogenase)Isovaleryl-CoA dehydrogenase (IVD) is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. The genetic deficiency of IVD results in an accumulation of isovaleric acid, which is toxic to the central nervous system and leads to isovaleric acidemia. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IVD. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Chicken, BovinePig (91%), Chicken (85%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACAD2 antibody FLJ12715 antibody FLJ34849 antibody IVD antibody isovaleryl-CoA dehydrogenase mitochondrial antibody isovaleryl Coenzyme A dehydrogenase antibody isovaleryl CoA dehydrogenase antibody isovaleryl-CoA dehydrogenase antibody |
|---|---|
| Molecular Weight(MW) | 46 kDa (note) (2) |
| Cellular Localization | Mitochondrion matrix |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using IVD antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using IVD antibody at 1:250 dilution.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000
Application
IVD antibody detects IVD protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10 % SDS-PAGE IVD antibody dilution: 1:1000
Application
Sample (30 ug of whole cell lysate) A: Hela B: JurKat 10% SDS PAGE diluted at 1:1000| Positive Control | HeLa , Jurkat , mouse brain , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
