Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IVD monoclonal antibody.Application
HEK293T cells transfected with either RC201077 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IVD antibody, and then analyzed by flow cytometry.Application
Anti-IVD mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with IVDApplication
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IVD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IVD.| Product Name | IVD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5A11] to IVDIsovaleryl-CoA dehydrogenase (IVD) is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. The genetic deficiency of IVD results in an accumulation of isovaleric acid, which is toxic to the central nervous system and leads to isovaleric acidemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Full length human recombinant protein of human IVD (NP_002612) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5A11 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| IHC 1:150 : |
|
| Species Reactivity | HumanDogMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IVD FLJ12715 FLJ34849 ACAD2 |
|---|---|
| Target | IVD |
| Tissue Specificity | is specific for human IVD (NP_002612). |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IVD monoclonal antibody.
Application
HEK293T cells transfected with either RC201077 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IVD antibody, and then analyzed by flow cytometry.
Application
Anti-IVD mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with IVD
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IVD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IVD.| Application Notes | IHC 1:150 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
