Application
JAB1 antibody [N1C1] detects JAB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: JAB1 protein stained by JAB1 antibody [N1C1] diluted at 1:200. Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] diluted at 1:20000. Blue: Hoechst 33342 staining.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000Application
JAB1 antibody [N1C1] detects JAB1 protein at nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. JAB1 antibody [N1C1] dilution: 1:500.| Product Name | JAB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to JAB1 (COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis))The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein is reported to be involved in the degradation of cyclin-dependent kinase inhibitor CDKN1B/p27Kip1. It is also known to be an coactivator that increases the specificity of JUN/AP1 transcription factors. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human JAB1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineRat (100%), Zebrafish (99%), Xenopus laevis (99%), Pig (100%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CSN5 antibody JAB1 antibody MGC3149 antibody MOV-34 antibody SGN5 antibody COPS5 antibody jun activation domain-binding protein 1 antibody signalosome subunit 5 antibody 38 kDa Mov34 homolog antibody COP9 signalosome complex subunit 5 antibody CO |
|---|---|
| Molecular Weight(MW) | 38 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
JAB1 antibody [N1C1] detects JAB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: JAB1 protein stained by JAB1 antibody [N1C1] diluted at 1:200. Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] diluted at 1:20000. Blue: Hoechst 33342 staining.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000
Application
JAB1 antibody [N1C1] detects JAB1 protein at nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. JAB1 antibody [N1C1] dilution: 1:500.| Positive Control | HeLa , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
