WB
Various lysates were subjected to SDS PAGE followed by western blot with AQP1 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with AQP1 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using AQP1 antibody at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AQP1 antibody at dilution of 1:400 and Omnimabs®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using AQP1 antibody at dilution of 1:800 and Multi-rAb Omnimabs ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | AQP1 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | ecombinant human AQP1 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IF-PIHCWB |
| WB:1:5000-1:20000 IHC:1:200-1:800 IF-P:1:200-1:800 |
|
| Species Reactivity | HumanMousePigRabbit |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | AQP1 |
|---|---|
| Gene Synonyms | CO CHIP28 AQP-CHIP |
| Gene Full Name | aquaporin 1 (Colton blood group) |
| Gene Summary | This gene encodes a small integral membrane protein with six bilayer spanning domains that functions as a water channel protein. This protein permits passive transport of water along an osmotic gradient. This gene is a possible candidate for disorders involving imbalance in ocular fluid movement. [provided by RefSeq, Aug 2016] |
| Alternative Names | Aquaporin 1 2E5D6 AQP 1 AQP-1 Aquaporin1 |
| Molecular Weight(MW) | 28 kDa, 38-40 kDa |
| Cellular Localization | Cell membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with AQP1 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with AQP1 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using AQP1 antibody at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AQP1 antibody at dilution of 1:400 and Omnimabs®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using AQP1 antibody at dilution of 1:800 and Multi-rAb Omnimabs ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:5000-1:20000 IHC:1:200-1:800 IF-P:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
