Application
JNK1 antibody detects JNK1 protein at cytoplasm on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. JNK1 antibody diluted at 1:500.Application
JNK1 antibody detects JNK1 protein at cytoplasm on mouse small intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse small intestine. JNK1 antibody diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using JNK1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | JNK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to JNK1 (mitogen-activated protein kinase 8)The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human JNK1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, BovineRat (99%), Xenopus laevis (94%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | JNK antibody JNK1 antibody JNK1A2 antibody JNK21B1/2 antibody PRKM8 antibody SAPK1 antibody MAPK8 antibody mitogen-activated protein kinase 8 antibody stress-activated protein kinase 1 antibody stress-activated protein kinase 1c antibody MAP kina |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (17) |
Application
JNK1 antibody detects JNK1 protein at cytoplasm on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. JNK1 antibody diluted at 1:500.
Application
JNK1 antibody detects JNK1 protein at cytoplasm on mouse small intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse small intestine. JNK1 antibody diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using JNK1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | 293T , A431 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
