Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-JNK1 monoclonal antibody.Application
JNK1 antibody ( 1C2 ) at 1:1000 + NIH/3T3 cell lysateApplication
Anti-JNK1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with JNK1Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JNK1.| Product Name | JNK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1C2] to JNK1Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 1-384 of human JNK1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 1C2 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC/IF (1:100): |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRKM8 JNK1A2 MAPK8 JNK21B1/2 SAPK1 JNK1 JNK |
|---|---|
| Target | JNK1 |
| Tissue Specificity | is specific for amino acids 1-384 of human JNK1 |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-JNK1 monoclonal antibody.
Application
JNK1 antibody ( 1C2 ) at 1:1000 + NIH/3T3 cell lysate
Application
Anti-JNK1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with JNK1
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JNK1.| Application Notes | ICC/IF (1:100): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
