| Product Name | JNK1/2/3 (phospho Thr183/Tyr185) antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to JNK1/2/3 (phospho Thr183/Tyr185)The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Sep 2008] |
| Modification | p-Thr183/Tyr185 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIP |
| Western blotting (1-2 µg/ml), immunoprecipitation (10-20 µg/ml), immunocytochemistry (10-20 µg/ml), and Immunohistochemistry (20-30 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the p46 and p54 JNK/SAPK dually phosphorylated at Thr183 and Tyr185.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SAPK phospho JNK T183/185 P54ASAPK JNK2B phospho T183/185 JNK JNK2BETA Phospho JNK (Thr183/185) MAPK9 Phospho JNK (pT183/185) JNK (pThr183/185) JNK2ALPHA JNK T183/185 phospho JNK (Phospho T183/185) PRKM9 JNK2A J |
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| Target | JNK1/2/3 (phospho Thr183/Thr185) |
| Tissue Specificity | This antibody is specific for JNK/SAPK (phospho Thr183/Tyr185). |
| Application Notes | Western blotting (1-2 µg/ml), immunoprecipitation (10-20 µg/ml), immunocytochemistry (10-20 µg/ml), and Immunohistochemistry (20-30 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the p46 and p54 JNK/SAPK dually phosphorylated at Thr183 and Tyr185.: |
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| Form | Liquid |
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| Storage Instructions | The antibody solution should be gently mixed before use. Store at -20C. Product should remain stable for at least 12 months. |
| Storage Buffer | PBS (pH 7.2) containing 50% glycerol, 1% BSA, 0.02% sodium azide |
