Application
A. Western blot analysis on Hek293 cell lysates using anti-JNK3 Phospho (pS223) RabMAb (cat. #) 1:20000 dilution. Cells were either (A) untreated (B) treated with UV.| Product Name | JNK1/2/3 (phospho Tyr185/Tyr223) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [EP1597Y] to JNK1/2/3 (phospho Tyr185/Tyr223)C-Jun N-terminal kinases (JNK) are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group consists of three isoforms (JNK1, 2, and 3) and regulates transcription activity of the AP-1 complex, and release and regulation of cytochome c . JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 . Unlike other JNK isoforms, JNK3 is only expressed in the heart and nervous system. JNK3 has been implicated in stress-induced neuronal apoptosis . |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding tyrosine 223 of human JNK3 was used as immunogen. |
| Modification | p-Tyr185/Tyr223 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP1597Y |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| For WB: Use at a dilution of 1:1,000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | PRKM10 JNK3 FLJ33785 JNK3A p54bSAPK MAPK10 FLJ12099 MGC50974 p493F12 JNK1 (Phospho) Phospho JNK1 |
|---|---|
| Target | JNK1/2/3 (phospho Tyr185/Tyr223) |
| Molecular Weight(MW) | 46 (note) |
| Tissue Specificity | The antibody will detect JNK1 (pY185), JNK2(pY185) and JNK3 (pY223). |
Application
A. Western blot analysis on Hek293 cell lysates using anti-JNK3 Phospho (pS223) RabMAb (cat. #) 1:20000 dilution. Cells were either (A) untreated (B) treated with UV.| Application Notes | For WB: Use at a dilution of 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
