Application
Immunofluorescence analysis of methanol-fixed HeLa, using JNK2 antibody at 1:200 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:2000Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hela 10% SDS PAGE diluted at 1:1000| Product Name | JNK2 antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to JNK2 (mitogen-activated protein kinase 9)The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human JNK2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | RatRat (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | JNK-55 antibody JNK2 antibody JNK2A antibody JNK2ALPHA antibody JNK2B antibody JNK2BETA antibody PRKM9 antibody SAPK antibody p54a antibody p54aSAPK antibody MAPK9 antibody stress-activated protein kinase 1a antibody MAP kinase 9 antibody stre |
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| Molecular Weight(MW) | 48 kDa (note) (3) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using JNK2 antibody at 1:200 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:2000
Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hela 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , mouse brain |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
