Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using JNK3 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:500| Product Name | JNK3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to JNK3 (mitogen-activated protein kinase 10)The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human JNK3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Chicken, Rhesus Monkey, BovineMouse (100%), Rat (100%), Zebrafish (97%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ12099 antibody FLJ33785 antibody JNK3 antibody JNK3A antibody MGC50974 antibody PRKM10 antibody p493F12 antibody p54bSAPK antibody MAPK10 antibody MAPK 10 antibody c-Jun N-terminal kinase 3 antibody stress-activated protein kinase JNK3 antibo |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) (8) |
| Cellular Localization | Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using JNK3 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:500| Positive Control | 293T , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
