Application
ChIP analysis was performed with extracts from HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes and JunB antibody. The precipitated DNA was detected by qPCR with primer set targeting to Exon1 or Exon28 of the LAMC1 gene.| Product Name | JunB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to JunBCellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation. The cellular oncogene families fos and jun encode nuclear proteins that can function as transcription factors. The fos family of nuclear oncogenes encode cFos, FosB, (fos-related antigen) Fra1, and Fra2. Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the ‘leucine-zipper’ motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes. |
| Immunogen | Synthetic peptide corresponding to residues D(45) P Y R G L K G P G A R G P G P E(61) of mouse JunB. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IPWBChIP |
| ChIP assay: 1-3 ul: |
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| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Gene Synonyms | Junb Jun-B oncogene |
|---|---|
| Target | JunB |
Application
ChIP analysis was performed with extracts from HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes and JunB antibody. The precipitated DNA was detected by qPCR with primer set targeting to Exon1 or Exon28 of the LAMC1 gene.| Application Notes | ChIP assay: 1-3 ul: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | lyophilized, purified rabbit IgG. Reconstitute with PBS. |
