| Product Name | JunD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to JunDJUND is the most broadly expressed member of the JUN family and the AP1 transcription factor complex. Weitzman et al. (2000) found that primary fibroblasts lacking murine Jund displayed p53-dependent growth arrest, upregulated p19(ARF) expression, and premature senescence. In contrast, immortalized cell lines lacking Jund showed increased proliferation and higher cyclin D1 levels. These properties were reminiscent of the effects of oncogenic RAS expression on primary and established cell cultures. Furthermore, Jund -/- fibroblasts exhibited increased p53-dependent apoptosis upon ultraviolet irradiation and were sensitive to the cytotoxic effects of tumor necrosis factor-alpha. The antiapoptotic role of JUND was confirmed using an in vivo model of TNF-mediated hepatitis. The authors proposed that JUND protects cells from senescence, or apoptotic responses to stress stimuli, by acting as a modulator of the signaling pathways that link RAS to p53. |
| Immunogen | Synthetic peptide: LGPPRLAALKDEPQT, corresponding to amino acids 231-245 of Human JunD. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/500 - 1/1000. Detects a band of approximately 50 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | JunD 3727 165162 P17535 |
|---|---|
| Target | JunD |
| Positive Control | Hela nuclear extract |
|---|---|
| Application Notes | WB: Use at a dilution of 1/500 - 1/1000. Detects a band of approximately 50 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide and 0.01% ThiomerosalConstituents: PBS containing 0.2% Gelatin |
