Western blot analysis of extracts from HeLa cells, treated with etoposide 25uM 24H, using Kallikrein-11 (Cleaved-Ile54) Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit polyclonal antibody to Kallikrein 11 (cleaved Ile54)Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues.
Immunogen
The antiserum was produced against synthesized peptide derived from human Kallikrein 11.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ELISA: 1:10000Optimal dilutions should be determined experimentally by the end user.:
Kallikrein-11 (Cleaved-Ile54) Antibody detects endogenous levels of fragment of activated Kallikrein-11 resulting from cleavage adjacent to Ile54.
Application
Application
Western blot analysis of extracts from HeLa cells, treated with etoposide 25uM 24H, using Kallikrein-11 (Cleaved-Ile54) Antibody. The lane on the right is treated with the synthesized peptide.
Application Notes
ELISA: 1:10000Optimal dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Store at -20 degrees Celcius. Product is stable for at least 1 year.
Storage Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.