Application
WB analysis of 293T and HepG2 lysates using KAP1 antibody at a dilution of 1:1,000.Application
ICC/IF analysis of HeLa cells using KAP1 antibody at a dilution of 1:200.Application
IHC-P analysis of human spleen tissue using KAP1 antibody.| Product Name | KAP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [4E1-D12-F8] to KAP1The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. |
| Immunogen | Purified recombinant human KAP1 protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4E1-D12-F8 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TIF1B RNF96 TF1B KAP1 tripartite motif containing 28 TRIM28 |
|---|---|
| Target | KAP1 / TIF1 beta |
| Molecular Weight(MW) | 100kDa (note) |
| Cellular Localization | Nucleus |
Application
WB analysis of 293T and HepG2 lysates using KAP1 antibody at a dilution of 1:1,000.
Application
ICC/IF analysis of HeLa cells using KAP1 antibody at a dilution of 1:200.
Application
IHC-P analysis of human spleen tissue using KAP1 antibody.| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol |
