Application
karyopherin alpha 2 antibody [C3], C-term detects karyopherin alpha 2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: karyopherin alpha 2 protein stained by karyopherin alpha 2 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample(30 µg of whole cell lysate) A:HeLa S3 B:Hep G2 C:MOLT4 10% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
karyopherin alpha 2 antibody [C3], C-term detects karyopherin alpha 2 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. karyopherin alpha 2 antibody [C3], C-term diluted at 1:250.| Product Name | karyopherin alpha 2 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to karyopherin alpha 2 (karyopherin alpha 2 (RAG cohort 1, importin alpha 1))The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human karyopherin alpha 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, ChimpanzeeMouse (100%), Rat (100%), Chimpanzee (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IPOA1 antibody QIP2 antibody RCH1 antibody SRP1alpha antibody KPNA2 antibody karyopherin subunit alpha-2 antibody SRP1-alpha antibody RAG cohort 1 antibody importin alpha 1 antibody importin subunit alpha-2 antibody RAG cohort protein 1 antibody |
|---|---|
| Target | karyopherin alpha 2 |
| Molecular Weight(MW) | 58 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
karyopherin alpha 2 antibody [C3], C-term detects karyopherin alpha 2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: karyopherin alpha 2 protein stained by karyopherin alpha 2 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample(30 µg of whole cell lysate) A:HeLa S3 B:Hep G2 C:MOLT4 10% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
karyopherin alpha 2 antibody [C3], C-term detects karyopherin alpha 2 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. karyopherin alpha 2 antibody [C3], C-term diluted at 1:250.| Positive Control | 293T , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
