Application
KDM3B antibody [C2C3], C-term immunoprecipitates KDM3B protein in IP experiments. IP samples: NT2D1 whole cell extract A. 50 μg NT2D1 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of KDM3B protein by 4 μg KDM3B antibody [C2C3], C-term 5 % SDS-PAGE The immunoprecipitated KDM3B protein was detected by KDM3B antibody [C2C3], C-term diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Sample (30 µg of whole cell lysate) A: NT2D1 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | KDM3B antibody [C2C3], C-term |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KDM3B (lysine (K)-specific demethylase 3B)Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human KDM3B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | MouseMouse (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 5qNCA antibody, C5orf7 antibody, JMJD1B antibody, KIAA1082 antibody, NET22 antibody, KDM3B antibody, lysine-specific demethylase 3B antibody, jumonji domain-containing protein 1B antibody, jmjC domain-containing histone demethylation protein 2B antibody, j |
|---|---|
| Molecular Weight(MW) | 192 kDa (note) (2) |
Application
KDM3B antibody [C2C3], C-term immunoprecipitates KDM3B protein in IP experiments. IP samples: NT2D1 whole cell extract A. 50 μg NT2D1 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of KDM3B protein by 4 μg KDM3B antibody [C2C3], C-term 5 % SDS-PAGE The immunoprecipitated KDM3B protein was detected by KDM3B antibody [C2C3], C-term diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Sample (30 µg of whole cell lysate) A: NT2D1 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
