Application
KDM6A antibody [N2C1], Internal detects KDM6A protein by western blot analysis. A. 30 µg U87-MG whole cell lysate/extract B. 30 µg SK-N-SH whole cell lysate/extract C. 30 µg IMR32 whole cell lysate/extract D. 30 µg SK-N-AS whole cell lysate/extract 5% SDS-PAGE KDM6A antibody [N2C1], Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using KDM6A antibody at 1:250 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using KDM6A antibody at 1:500 dilution.Application
KDM6A antibody [N2C1], Internal detects KDM6A protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. KDM6A antibody [N2C1], Internal dilution: 1:1000.Application
KDM6A antibody [N2C1], Internal immunoprecipitates KDM6A protein in IP experiments. IP samples: SK-N-SH whole cell extract A. 30 μg SK-N-SH whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of KDM6A protein by 4 μg KDM6A antibody [N2C1], Internal 5 % SDS-PAGE The immunoprecipitated KDM6A protein was detected by KDM6A antibody [N2C1], Internal diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-KDM6A antibody. The precipitated DNA was detected by PCR with primer set targeting to RBL1 promoter.| Product Name | KDM6A antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KDM6A (lysine (K)-specific demethylase 6A)This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human KDM6A. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPICC/IFIHC-PIPWB |
| WB:1:500~1:1000 IP:1:100~1:500 ICC/IF:1:100~1:500 IHC:1:50~1:100 *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Xenopus laevis, Dog, BovineXenopus laevis (82%), Dog (86%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686A03225 antibody MGC141941 antibody UTX antibody bA386N14.2 antibody KDM6A antibody ubiquitously-transcribed X chromosome tetratricopeptide repeat protein antibody bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat prot |
|---|---|
| Molecular Weight(MW) | 154 kDa (note) (7) |
| Cellular Localization | Nucleus |
Application
KDM6A antibody [N2C1], Internal detects KDM6A protein by western blot analysis. A. 30 µg U87-MG whole cell lysate/extract B. 30 µg SK-N-SH whole cell lysate/extract C. 30 µg IMR32 whole cell lysate/extract D. 30 µg SK-N-AS whole cell lysate/extract 5% SDS-PAGE KDM6A antibody [N2C1], Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using KDM6A antibody at 1:250 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using KDM6A antibody at 1:500 dilution.
Application
KDM6A antibody [N2C1], Internal detects KDM6A protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. KDM6A antibody [N2C1], Internal dilution: 1:1000.
Application
KDM6A antibody [N2C1], Internal immunoprecipitates KDM6A protein in IP experiments. IP samples: SK-N-SH whole cell extract A. 30 μg SK-N-SH whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of KDM6A protein by 4 μg KDM6A antibody [N2C1], Internal 5 % SDS-PAGE The immunoprecipitated KDM6A protein was detected by KDM6A antibody [N2C1], Internal diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-KDM6A antibody. The precipitated DNA was detected by PCR with primer set targeting to RBL1 promoter.| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS |
|---|---|
| Application Notes | WB:1:500~1:1000 IP:1:100~1:500 ICC/IF:1:100~1:500 IHC:1:50~1:100 *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
