Application
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE diluted at 1:1000Application
KEAP1 antibody [C1C3] detects KEAP1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KEAP1 protein stained by KEAP1 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | KEAP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to KEAP1 (kelch-like ECH-associated protein 1)This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human KEAP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Chimpanzee, BovineMouse (96%), Rat (96%), Pig (99%), Chimpanzee (100%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | INrf2 antibody KIAA0132 antibody KLHL19 antibody MGC10630 antibody MGC1114 antibody MGC20887 antibody MGC4407 antibody MGC9454 antibody KEAP1 antibody cytosolic inhibitor of Nrf2 antibody kelch-like ECH-associated protein 1 antibody kelch-like p |
|---|---|
| Target | KEAP1 |
| Molecular Weight(MW) | 70 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE diluted at 1:1000
Application
KEAP1 antibody [C1C3] detects KEAP1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KEAP1 protein stained by KEAP1 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | NT2D1 , IMR32 , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
