Application
Western blot analysis using recombinant human KGF. Lane1:10 ng Lane2: 50 ng Lane 3:100 ng| Product Name | KGF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to KGFKeratinocyte Growth Factor (KGF or FGF-7) is a potent mitogen for keratinocytes and epithelial cells. |
| Immunogen | Highly pure (>98%) recombinant hKGF (human Keratinocyte Growth Factor). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Unknown |
| Host Species | Rabbit |
| Tested Applications | ELISA,WB |
| ELISA:To detect KGF by direct ELISA (using 100μl/well antibody solution) a concentration of at least 2-3μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant KGF. Western Blot:To detect KGF by Western Blot analysis this antibody can be used at a concentration of 0.5-1 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant KGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HBGF-7, KGF, HBGF 7, HBGF7 |
|---|---|
| Target | KGF |
| Molecular Weight(MW) | 19 (note) |
Application
Western blot analysis using recombinant human KGF. Lane1:10 ng Lane2: 50 ng Lane 3:100 ng| Application Notes | ELISA:To detect KGF by direct ELISA (using 100μl/well antibody solution) a concentration of at least 2-3μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant KGF. Western Blot:To detect KGF by Western Blot analysis this antibody can be used at a concentration of 0.5-1 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant KGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 50% glycerol and 0.02% thimerosal |
