Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using KHS antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using KHS antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE MAP4K5 antibody diluted at 1:5000Application
Immunoprecipitation of KHS protein from 293T whole cell extracts using 5 μg of KHS antibody . Western blot analysis was performed using KHS antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | KHS antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to KHS (mitogen-activated protein kinase kinase kinase kinase 5)This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human KHS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (94%), Rat (94%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GCKR antibody, KHS antibody, KHS1 antibody, MAPKKKK5 antibody, MAP4K5 antibody, MEK kinase kinase 5 antibody, kinase homologous to SPS1/STE20 antibody, MEKKK 5 antibody, MAPK/ERK kinase kinase kinase 5 antibody, germinal center kinase-related antibody, mit |
|---|---|
| Target | KHS |
| Molecular Weight(MW) | 95 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using KHS antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using KHS antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE MAP4K5 antibody diluted at 1:5000
Application
Immunoprecipitation of KHS protein from 293T whole cell extracts using 5 μg of KHS antibody . Western blot analysis was performed using KHS antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
