Application
KHSRP antibody detects KHSRP protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KHSRP antibody diluted at 1:1000.Application
KHSRP antibody detects KHSRP protein at nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KHSRP protein stained by KHSRP antibody diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
KHSRP antibody detects KHSRP protein at cytoplasm and nucleus in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. KHSRP antibody diluted at 1:500.Application
KHSRP antibody detects KHSRP protein at cytoplasm and nucleus in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. KHSRP antibody diluted at 1:500.Application
KHSRP antibody detects KHSRP protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KHSRP antibody diluted at 1:1000.| Product Name | KHSRP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KHSRP (KH-type splicing regulatory protein)The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes, including transcription, alternative pre-mRNA splicing, and mRNA localization (Min et al., 1997 [PubMed 9136930]; Gherzi et al., 2004 [PubMed 15175153]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human KHSRP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | ChickenChicken (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KH-type splicing regulatory protein , FUBP2 , FBP2 , KSRP |
|---|---|
| Target | KHSRP |
| Molecular Weight(MW) | 73 kDa (note) |
| Cellular Localization | Nucleus (By similarity) , Cytoplasm (By similarity) |
Application
KHSRP antibody detects KHSRP protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KHSRP antibody diluted at 1:1000.
Application
KHSRP antibody detects KHSRP protein at nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KHSRP protein stained by KHSRP antibody diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
KHSRP antibody detects KHSRP protein at cytoplasm and nucleus in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. KHSRP antibody diluted at 1:500.
Application
KHSRP antibody detects KHSRP protein at cytoplasm and nucleus in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. KHSRP antibody diluted at 1:500.
Application
KHSRP antibody detects KHSRP protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KHSRP antibody diluted at 1:1000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
