Application
WB analysis of HepG2 cells using KIAA0427 antibody at 0.2-1μg/ml.| Product Name | KIAA0427 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KIAA0427CTIF is a component of the CBP80 (NCBP1; MIM 600469)/CBP20 (NCBP2; MIM 605133) translation initiation complex that binds cotranscriptionally to the cap end of nascent mRNA. The CBP80/CBP20 complex is involved in a simultaneous editing and translation step that recognizes premature termination codons (PTCs) in mRNAs and directs PTC-containing mRNAs toward nonsense-mediated decay (NMD). On mRNAs without PTCs, the CBP80/CBP20 complex is replaced with cytoplasmic mRNA cap-binding proteins, including EIF4G (MIM 600495), and steady-state translation of the mRNAs resumes in the cytoplasm (Kim et al., 2009 [PubMed 19648179]).[supplied by OMIM, Dec 2009] |
| Immunogen | A synthetic peptide corresponding to a N-terminal region of Human KIAA0427 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Cow: 93%; Dog: 93%; Guinea Pig: 79%; Human: 100%; Mouse: 93%; Pig: 93%; Rabbit: 77% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KIAA0427 , CTIF , GM672 |
|---|---|
| Target | KIAA0427 |
| Molecular Weight(MW) | 68 KDa (note) |
Application
WB analysis of HepG2 cells using KIAA0427 antibody at 0.2-1μg/ml.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
