Application
Immunocytochemistry/Immunofluorescence: Kif2a Antibody [ - Staining of HeLa cells fixed in 3.5% paraformaldehyde. Centrosome staining during interphase and spindle pole staining during mitosis.| Product Name | Kif2a antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Kif2aKif2a is a kinesin-like motor protein that depolymerizes microtubules. It is found in several vertebrate cell types but is strongly expressed in neuronal cells. In dividing cells it is required for bipolar spindle formation and chromosome segregation. In neuronal cells it is involved in neurite outgrowth. |
| Immunogen | Produced by immunizing rabbits with a recombinant segment of the N-terminal domain of the human protein. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| This antibody may be used for Western blotting, immunoprecipitation, and immunofluorescence. A band at ~110 kDa can be detected by Western blot. Other applications have not been tested. Suggested working dilutions: * Western Blot - 1:10,000 Immunoprecipitation - 1:10,000 Immunofluorescence - 1:10,000 *The investigator should determine the optimal working dilution for a specific application. | |
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HK2, KIF2, Kinesin like protein KIF2, Kinesinlike protein KIF2 |
|---|---|
| Target | Kif2a |
| Tissue Specificity | This antibody is specific for Kif2a. |
Application
Immunocytochemistry/Immunofluorescence: Kif2a Antibody [ - Staining of HeLa cells fixed in 3.5% paraformaldehyde. Centrosome staining during interphase and spindle pole staining during mitosis.| Application Notes | This antibody may be used for Western blotting, immunoprecipitation, and immunofluorescence. A band at ~110 kDa can be detected by Western blot. Other applications have not been tested. Suggested working dilutions: * Western Blot - 1:10,000 Immunoprecipitation - 1:10,000 Immunofluorescence - 1:10,000 *The investigator should determine the optimal working dilution for a specific application. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
