Application
KIF4A antibody detects KIF4A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KIF4A antibody diluted by 1:1000.Application
Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using KIF4A antibody at 1:500 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using KIF4A antibody at 1:200 dilution.| Product Name | KIF4A antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to KIF4A (kinesin family member 4A)Kinesins, such as KIF4A, are microtubule-based motor proteins that generate directional movement along microtubules. They are involved in many crucial cellular processes, including cell division (Zhu and Jiang, 2005 [PubMed 15625105]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human KIF4A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Xenopus laevis, Chicken, BovineXenopus laevis (81%), Chicken (82%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ12530 antibody, FLJ12655 antibody, FLJ14204 antibody, FLJ20631 antibody, KIF4 antibody, KIF4G1 antibody, KIF4A antibody, chromokinesin-A antibody, chromosome-associated kinesin KIF4A antibody, kinesin family member 4A antibody |
|---|---|
| Molecular Weight(MW) | 140 kDa (note) (2) |
| Cellular Localization | Nucleus matrix |
Application
KIF4A antibody detects KIF4A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KIF4A antibody diluted by 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using KIF4A antibody at 1:500 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using KIF4A antibody at 1:200 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
